Fixation for QPS

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• Specimen
  • If there are dense ciliates in the fresh sample sea water, then use the sample sea water as a specemen.
  • In case, if the density of ciliates are not enough high, keep the sample sea water at about 20 C for a week under L/D=12/12 illumination, then some of them will grow and will become dense.
    
• 1:1 mixture with Bouin's fluid
  • 50 ml Bouin's fixative could be mixed with the same amount of sample sea water immediatelly.
  • Stay the mixture at least over night to get the well fixed cells.

( Lower concentration of Bouin's fluid, which was mentioned by Lynn (1992) as the use of "concentrated Bouin's fluid", did not work well in case of my specimens.)

QuickTime Movie of this procedure :(800 kbytes)